Fast-food chain Zensho Co. stopped serving "gyudon" -- bowls of rice topped with seasoned beef -- Thursday after it exhausted its beef inventory following the government's ban on U.S. beef.

Zensho, a Tokyo-based firm that runs 480 Sukiya restaurants across Japan, is the second major gyudon chain to remove beef dishes from its menu, following Nakau Co., which stopped serving them Monday.

"I always want to eat gyudon. I hope they will resume serving it at an early date," one customer said after eating a gyudon bowl at a Sukiya eatery in Tokyo's Minato Ward.